• 细小试纸使用方法
  • 本站编辑:杭州爱谨生物科技有限公司发布日期:2019-08-08 10:47 浏览次数:

1.检测样品

1)以新鲜的粪便做为检测样品

2)采集样品时有未知的感染性,故必须由经过培训或专业的技术人员来操作

2.检测方法

1)检测前的准备:存放在冰箱里的检测试纸,检测前15-30分钟应放在室温中,使之温度与室温一致。检测样品在比室温低的状态下开封容器时,由于结成水滴使试纸湿度升高,故要特别注意。

2)在室温中保存的试纸,可以直接使用。

3)用采样棒在粪便的表面与内部多面采集,或从检测犬的肛门直接采集。

4)将采集样品的采样棒放入收集管中使之浸泡于缓冲液中,充分摇晃。

5)从铝箔包装袋中取出试纸置于干燥而水平的表面。

6)拧开缓冲液的输出口,向样品孔缓慢而准确地滴入3滴检测液。

7)等待检测液在吸收衬垫上扩散完毕。

8)约5-10分钟后,对照线(C)变为红色时可以判断结果。反应时间越长,对照线与检测线的色带越深,故对照线完全变为红色之后在一定时间内判断可以得到更为准确的结果。

3.结果判断

通过检测线与对照线是否出现色带,来判断结果。无论检测样品中有无犬细小病毒抗原对照线(C)都会显示出色带,如不出现色带,要考虑到操作是否有误,或试剂是否有问题应重新检测。检测线上是否出现色带来判断检测样品中是否含有犬细小病毒抗原。

1)阴性结果检测线(T)没有出现色带,而对照线(C)出现色带,判断为阴性。

2)检测线(T)对照线(C)都出现色带,判断为阳性。

3)无结果检测线(T)对照线(C)都不出现色带,或只出现检测线(T)色带判断为无效果。

A.要考虑到操作是否有误或试剂是否有问题,应重新检测。

B.检测样品中犬瘟热抗原的效价特别高时,检测线颜色深而对照线颜色浅,这时需要将检测样品稀释10倍后再检验,这样可以得到检测线(T)与对照线(C)都出现色带的正确结果。

1. Test samples

1) fresh feces were used as test samples

2) the sample is infectious and unknown, so it must be operated by trained or professional technicians

2. Detection method

1) preparation before test: test paper stored in the refrigerator should be kept at room temperature for 15-30 minutes before test, so that the temperature is consistent with room temperature. When the test sample is opened at a lower temperature than room temperature, special attention should be paid to the moisture content of the test paper increased due to the formation of water droplets.

2) test paper kept at room temperature can be used directly.

3) collect the samples on the surface and inside of the feces, or directly from the anus of the detection dog.

4) put the sampling rod of the sample into the collecting tube and soak it in the buffer, shaking it fully.

5) remove the test paper from the aluminum foil package and place it on a dry, horizontal surface.

6) unscrew the buffer outlet and slowly and accurately drop 3 drops of the test solution into the sample hole.

7) wait for the test solution to be diffused on the absorption pad.

8) the results can be judged when the control line (C) turns red after about 5-10 minutes. The longer the reaction time, the darker the color bands of the control line and the detection line will be. Therefore, more accurate results can be obtained after the control line completely turns red in a certain period of time.

3. Result judgment

Through the detection line and control line whether there is a ribbon, to judge the results. Whether or not canine parvovirus antigen control wire (C) is present in the test sample, it will show excellent bands. If no bands are present, it should be retested considering whether the operation is wrong or the reagent is wrong. The presence of color bands on the detection line determines whether the samples contain canine parvovirus antigen.

1) no color bands appear on the test line (T) with negative results, while the color bands appear on the control line (C) with negative results.

2) color bands appear on both the test line (T) and the control line (C), which are judged to be positive.

3) no color bands appear in the control line (C) or only the color bands appear in the test line (T) are judged to have no effect.

A. retesting should be carried out to consider whether the operation is faulty or the reagent is defective.

B. When the titer of canine distemper antigen in the test sample is particularly high, the color of the detection line is dark and the color of the control line is light. At this time, it is necessary to dilute the test sample by 10 times before testing, so as to obtain the correct result that both the detection line (T) and the control line (C) have color bands.