1. Test samples
1) fresh feces were used as test samples
2) the sample is infectious and unknown, so it must be operated by trained or professional technicians
2. Detection method
1) preparation before test: test paper stored in the refrigerator should be kept at room temperature for 15-30 minutes before test, so that the temperature is consistent with room temperature. When the test sample is opened at a lower temperature than room temperature, special attention should be paid to the moisture content of the test paper increased due to the formation of water droplets.
2) test paper kept at room temperature can be used directly.
3) collect the samples on the surface and inside of the feces, or directly from the anus of the detection dog.
4) put the sampling rod of the sample into the collecting tube and soak it in the buffer, shaking it fully.
5) remove the test paper from the aluminum foil package and place it on a dry, horizontal surface.
6) unscrew the buffer outlet and slowly and accurately drop 3 drops of the test solution into the sample hole.
7) wait for the test solution to be diffused on the absorption pad.
8) the results can be judged when the control line (C) turns red after about 5-10 minutes. The longer the reaction time, the darker the color bands of the control line and the detection line will be. Therefore, more accurate results can be obtained after the control line completely turns red in a certain period of time.
3. Result judgment
Through the detection line and control line whether there is a ribbon, to judge the results. Whether or not canine parvovirus antigen control wire (C) is present in the test sample, it will show excellent bands. If no bands are present, it should be retested considering whether the operation is wrong or the reagent is wrong. The presence of color bands on the detection line determines whether the samples contain canine parvovirus antigen.
1) no color bands appear on the test line (T) with negative results, while the color bands appear on the control line (C) with negative results.
2) color bands appear on both the test line (T) and the control line (C), which are judged to be positive.
3) no color bands appear in the control line (C) or only the color bands appear in the test line (T) are judged to have no effect.
A. retesting should be carried out to consider whether the operation is faulty or the reagent is defective.
B. When the titer of canine distemper antigen in the test sample is particularly high, the color of the detection line is dark and the color of the control line is light. At this time, it is necessary to dilute the test sample by 10 times before testing, so as to obtain the correct result that both the detection line (T) and the control line (C) have color bands.